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Curr Opin Biotechnol. 2000 Oct;11(5):434-9.

Detection of genes essential in specific niches by signature-tagged mutagenesis.

Author information

1
Microbiologie Moléculaire et Génie des Protéines, Pavillon Charles-Eugène Marchand et Faculté de Médecine, Université Laval, Sainte-Foy, G1K 7P4, Québec, Canada.

Abstract

Variations of the signature-tagged mutagenesis (STM) technique are now possible and the method can be applied to most pathogens that have an STM-selectable phenotype in a host system. STM screening of 15,040 mutants from 11 bacterial species identified 323 in vivo attenuated mutants. As a genome-scanning tool, STM will yield information about genes with unknown functions as well as information crucial for understanding microbial pathogenesis.

PMID:
11024359
[Indexed for MEDLINE]

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