Send to

Choose Destination
Curr Opin Biotechnol. 2000 Oct;11(5):434-9.

Detection of genes essential in specific niches by signature-tagged mutagenesis.

Author information

Microbiologie Moléculaire et Génie des Protéines, Pavillon Charles-Eugène Marchand et Faculté de Médecine, Université Laval, Sainte-Foy, G1K 7P4, Québec, Canada.


Variations of the signature-tagged mutagenesis (STM) technique are now possible and the method can be applied to most pathogens that have an STM-selectable phenotype in a host system. STM screening of 15,040 mutants from 11 bacterial species identified 323 in vivo attenuated mutants. As a genome-scanning tool, STM will yield information about genes with unknown functions as well as information crucial for understanding microbial pathogenesis.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center