Format

Send to

Choose Destination
Biophys J. 2000 Oct;79(4):2199-208.

The use of pHluorins for optical measurements of presynaptic activity.

Author information

1
Department of Biochemistry, The Weill Medical College of Cornell University, New York, New York 10021, USA.

Abstract

Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK approximately 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.

PMID:
11023924
PMCID:
PMC1301110
DOI:
10.1016/S0006-3495(00)76468-X
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center