Send to

Choose Destination
Cereb Cortex. 2000 Oct;10(10):974-80.

Serotonin induces EPSCs preferentially in layer V pyramidal neurons of the frontal cortex in the rat.

Author information

Interdepartmental Neuroscience Program, Section of Neurobiology, and Departments of Psychiatry and Pharmacology, Yale University School of Medicine, New Haven, CT, USA.


The effect of serotonin (5-HT) on the release of glutamate was examined in pyramidal cells in layers II-VI of the frontal cortex. The intracellular recording electrode contained 1% biocytin so the neurons could later be visualized with an avidin-biotin peroxidase method. Pyramidal cells in layer V of the frontal cortex showed the greatest 5-HT-induced increase in both the frequency and amplitude of 'spontaneous' (non-electrically evoked) excitatory post-synaptic currents (EPSCs). A small proportion of neurons in layer II/III showed an increase in EPSC frequency, whereas cells in layer VI showed no significant change in either EPSC frequency or amplitude. The physiological response to 5-HT mirrors the high density of 5-HT(2A) receptors in layer V, as well as the pattern of thalamic projections in frontal cortex. The specific induction of EPSCs in layer V neurons suggests that 5-HT preferentially modulates the output neurons of the frontal cortex.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center