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Biochem Biophys Res Commun. 2000 Sep 16;276(1):71-6.

Enzymes leading to the nucleotide sugar precursors for exopolysaccharide synthesis in Burkholderia cepacia.

Author information

1
Centro de Engenharia Biológica e Quimica, Instituto Superior Técnico, Av. Rovisco Pais, Lisboa, 1049-001, Portugal.

Abstract

Based on the chemical composition of the exopolysaccharide produced by the cystic fibrosis bacterial isolate Burkholderia cepacia IST408, we postulated and confirmed, based on the specificity of enzymes detected in crude cell-free extracts, the pathway leading to the presumptive activated sugar precursors: UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid, GDP-D-mannose, and GDP-D-rhamnose. Results also suggest that regulation of the expression of the mucoid phenotype in B. cepacia does not occur at the level of synthesis of any of these enzymes.

PMID:
11006084
DOI:
10.1006/bbrc.2000.3438
[Indexed for MEDLINE]

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