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Mol Cell Endocrinol. 2000 Sep 25;167(1-2):43-53.

A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer.

Author information

1
Geriatric Research, Education, and Clinical Center and Research Service, Veterans Affairs Medical Center, Miami, FL 33125, USA. cpsma@msn.com

Abstract

The transgenic mouse line Ggamma/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene.

PMID:
11000519
[Indexed for MEDLINE]

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