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Microb Drug Resist. 2000 Summer;6(2):111-7.

Evidence of an efflux pump in Serratia marcescens.

Author information

1
Microbiology Unit, University of Barcelona, Spain.

Abstract

Spontaneous mutants resistant to fluoroquinolones were obtained by exposing Serratia marcescens NIMA (wild-type strain) to increasing concentrations of ciprofloxacin both in liquid and on solid media. Frequencies of mutation ranged from 10(-7) to 10(-9). Active expulsion of antibiotic was explored as a possible mechanism of resistance in mutants as well as changes in topoisomerase target genes. The role of extrusion mechanisms in determining the emergence of multidrug-resistant bacteria was also examined. Mutants resistant to high concentrations of fluoroquinolones had a single mutation in their gyrA QRDR sequences, whereas the moderate resistance in the rest of mutants was due to extrusion of the drug.

PMID:
10990265
DOI:
10.1089/107662900419410
[Indexed for MEDLINE]

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