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Anim Reprod Sci. 2000 Nov 1;63(3-4):221-9.

Synthesis of 60- and 72 kDa heat shock proteins in early porcine embryogenesis.

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Department of Comparative Medicine, Pig Research Institute Taiwan, P. O. Box 23, Chunan, 35099, ROC, Miaoli, Taiwan.


Proteins of selected embryonic stages were metabolically labeled with [(35)S]-methionine and analyzed by two-dimensional SDS-polyacrylamide gel electrophoresis (2-D PAGE) to study protein expression from 4- to 8-cell to blastocyst stage of porcine embryos. Two proteins with molecular weights of 60 and 72kDa were de novo synthesized during the 4- to 8-cell stage were the earliest that were detected. They were identified as HSP60 and HSP72 according to their locations on 2-D autoradiography and the immunoblotting result of anti-HSP 60 and HSP 72 antibodies of 1-cell stage of porcine embryos. In protein translation in early pig embryogenesis the timing of their synthesis suggests that HSP60 and HSP72 play significant roles as chaperones.

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