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Mol Biotechnol. 2000 Jul;15(3):249-57.

Adaptation of mammalian cells to growth in serum-free media.

Author information

1
Genetic Institute, Andover, MA 01810, USA.

Abstract

A three-step protocol is described for adapting an anchorage-dependent, serum-dependent recombinant mammalian cell lineage to high density serum-free suspension culture. The objective is a cell lineage that is well-suited for the manufacture of a recombinant protein. The first step of the protocol generates an anchorage-independent cell lineage by culturing trypsin-treated cells in spinner flasks using serum-containing medium. The second step adapts the lineage to serum-free medium through a series of serum reduction steps in the presence of defined growth-promoting additives. The third step adapts the lineage to high-cell-density conditions by culturing the cells in a bioreactor in a manner that allows development of tolerance to growth-inhibiting substances released by the cells. Examples are presented for the use of this protocol for recombinant CHO cells.

PMID:
10986701
DOI:
10.1385/MB:15:3:249
[Indexed for MEDLINE]

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