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FEBS Lett. 2000 Aug 18;479(3):127-30.

Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence.

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1
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.

Abstract

A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm. Using the remarkable similarity of the drFP583 and dsFP593 genes, we performed a 'shuffling' procedure to generate a pool of mutants consisting of various combinations of parts of both genes. One 'hybrid gene' was chosen for subsequent random mutagenesis, which resulted in a mutant variant with a uniquely red-shifted emission maximum at 616 nm.

PMID:
10981720
DOI:
10.1016/s0014-5793(00)01895-0
[Indexed for MEDLINE]
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