Format

Send to

Choose Destination
See comment in PubMed Commons below
Forensic Sci Int. 2000 Sep 11;113(1-3):103-7.

Application of mtDNA sequence analysis in forensic casework for the identification of human remains.

Author information

1
Institute of Legal Medicine, Johannes Gutenberg University, Mainz, Germany. kbender@mail.uni-mainz.de

Abstract

In four forensic cases of unidentified skeletal remains investigated in the last year, we were able to attach three to missing persons. In one case we could show that the discovered bone sample did not fit to a missing child. The method for mitochondrial DNA analysis for the routine identification of skeletal remains was established in our institute by typing bone samples of defined age obtained from Frankfurt's cemetery. Reproducible results were obtained for bones up to 75 years old. For analysis the bone samples were pulverised to fine powder, decalcified and DNA was extracted. From the DNA we amplified a 404-bp fragment from HV-1 and a 379-bp fragment from HV-2 of the mtDNA control region. After sequencing of the PCR products, the results were compared to the Anderson reference sequence and to putative maternal relatives.

PMID:
10978609
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center