[Cloning of taxadiene synthase cDNA from the cell line of Taxus cuspidata]

Sheng Wu Gong Cheng Xue Bao. 2000 Mar;16(2):158-60.
[Article in Chinese]

Abstract

Taxadiene synthase plays an important role in taxol biosynthesis. RT-PCR was used for cloning taxadiene synthase cDNA fragment from the cells of T. cuspidata. The cDNA was cloned into vector pGEM and transformed to E. coli J M109. The cloned cDNA named pCBMZ was further confirmed by Southern blotting assay and was sequenced. The result showed that taxadiene synthase cDNA of Taxus cuspidata was highly homologous with that of Taxus brevifolia.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification*
  • Isomerases / genetics*
  • Molecular Sequence Data
  • Plants, Medicinal / enzymology*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA, Complementary
  • Isomerases
  • taxa-4(5),11(12)-diene synthase