Abstract
The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antigens, Viral / analysis
-
Blotting, Southern
-
Caliciviridae / classification*
-
Caliciviridae / genetics
-
Caliciviridae / immunology*
-
Caliciviridae Infections / virology*
-
Capsid / genetics*
-
Capsid / metabolism
-
Cloning, Molecular
-
Enzyme-Linked Immunosorbent Assay / methods
-
Feces / virology
-
Gastroenteritis / virology*
-
Humans
-
Molecular Sequence Data
-
Norwalk virus / classification
-
Norwalk virus / genetics
-
Norwalk virus / immunology
-
Rabbits
-
Recombinant Proteins / immunology
-
Reverse Transcriptase Polymerase Chain Reaction
Substances
-
Antigens, Viral
-
Recombinant Proteins