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Virus Res. 2000 Jul;68(2):99-107.

Construction of a stable and highly infectious intron-containing cDNA clone of plum pox potyvirus and its use to infect plants by particle bombardment.

Author information

1
Centro Nacional de Biotecnología, Campus de la Universidad Autónoma, CSIC, Cantoblanco 28049, Madrid, Spain.

Abstract

An infectious plum pox potyvirus cDNA clone was constructed placing a copy of the full-length sequence of the virus genome between an enhanced cauliflower mosaic virus 35S promoter and a nopaline synthase termination signal. Stabilization of the clone and faster growth of bacteria, in addition to higher plasmid yield, followed a modification consisting of the insertion of an intron which interrupted the viral open reading frame at the P3 region. This intron-containing clone was infectious when inoculated into plants after undergoing in vivo transcription and splicing. Particle bombardment delivery of the cDNA greatly increased the efficiency of plant infection.

PMID:
10958981
[Indexed for MEDLINE]

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