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Anal Chem. 2000 Aug 1;72(15):3678-81.

Single-molecule analysis of DNA immobilized on microspheres.

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Department of Chemistry, University of Cambridge, UK.


The formation and analysis of single molecules of fluorescently labeled DNA immobilized on polystyrene microspheres is described. Analysis by confocal fluorescence microscopy revealed single-step photobleaching, characteristic of a single fluorophore. Microspheres provide a means of locating single molecules by bright-field microscopy, prior to single-molecule detection. This allows the interrogation of single molecules without suffering the limitations of premature photobleaching. Statistical analysis of fluorescence intensities for >100 microspheres suggests attachment of DNA to micropsheres to be consistent with Poisson statistics.

[Indexed for MEDLINE]

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