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J Gen Virol. 2000 Sep;81(Pt 9):2161-6.

Pathogen-specific resistance to Rift Valley fever virus infection is induced in mosquito cells by expression of the recombinant nucleoprotein but not NSs non-structural protein sequences.

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  • 1Groupe des Bunyavirid├ęs, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.


Rift Valley fever virus (RVFV) is an arbovirus of the BUNYAVIRIDAE: family, causing recurrent disease outbreaks in Africa. Natural vertebrate hosts include cattle and humans. Several mosquito species belonging to the AEDES: and CULEX: genera act as vectors of this phlebovirus. To test whether pathogen-derived resistance against RVFV could be induced by expressing genomic sequences in mosquito cells, as has been shown for La Crosse and dengue 2 viruses, we generated various recombinant Semliki Forest viruses expressing the S segment (or its genes) in the genomic or antigenomic sense. Expression of the N but not the NSs gene interfered with the production of RVFV in mosquito cells and this phenomenon was RNA- but not protein-dependent. These results raise questions on the molecular mechanisms involved in virus resistance.

[PubMed - indexed for MEDLINE]
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