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Mol Cell. 2000 Jul;6(1):65-75.

ARK-1 inhibits EGFR signaling in C. elegans.

Author information

1
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom. nah@mrc-lmb.cam.ac.uk

Abstract

A screen for synthetic enhancers of sli-1 identified ark-1 (forAck-related tyrosine kinase), a novel inhibitor of let-23 EGFR signaling in C. elegans. An ark-1 mutation synergizes with mutations in other negative regulators of let-23, resulting in increased RAS signaling. Genetic analysis suggests that ARK-1 acts upstream of RAS and is dependent upon SEM-5. ARK-1 inhibits LET-23-mediated ovulation, a RAS-independent function. ARK-1 physically interacts with SEM-5 in the yeast two-hybrid assay. We find that sem-5 also has a negative function in let-23-mediated ovulation and suggest that this negative function is mediated by the recruitment of inhibitors such as ARK-1.

PMID:
10949028
[Indexed for MEDLINE]
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