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New Microbiol. 2000 Jan;23(1):11-20.

Application of PFGE performed with XbaI to an epidemiological and phylogenetic study of Salmonella serotype typhimurium. Relations between genetic types and phage types.

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Departamento de Biología Funcional, Universidad de Oviedo, Facultad de Medicina, Spain.


PFGE performed with XbaI was evaluated and applied as a typing method in a series of 68 Salmonella serotype Typhimurium strains collected in a Spanish region throughout 1984-1994, and four reference strains. Using bands > 100 kb as a separation criterion, 26 pulsed-field profiles (PFPs) were defined, with a discrimination index of 0.87. The XbaI-profiles were grouped into three clusters and two additional branches in a dendrogram of similarity (significance level = 0.7). Strains belonging to PFP-X1 and PFP-X2 predominated (26.4% and 23.6%, respectively) and fell into the major cluster. The series had been previously analysed by phage typing and a three-way ribotyping procedure, and a certain degree of relation among the three methods was revealed, PFGE being the most discriminatory. Combining data from the three methods a further differentiation into 46 clonal lines was obtained, and four lines, at least, could be considered as endemic in the region under study. This procedure proved to be a powerful epidemiological tool for characterization of Typhimurium and in the investigation of outbreaks.

[Indexed for MEDLINE]

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