Cotranscription of 5S rRNA-tRNA(Arg)(ACG) from Brassica napus chloroplasts and processing of their intergenic spacer

Gene. 2000 Aug 8;253(2):303-11. doi: 10.1016/s0378-1119(00)00234-1.

Abstract

S1 mapping showed that at least a significant portion of the 5S rRNA and tRNA(Arg)(ACG) is co-transcribed in canola chloroplast, making trnR the last gene transcribed in an operon of which the final sequence is 5'-16S-tRNA(Ile)-tRNA(Ala)-23S-4.5S-5S-tRNA(Arg)-3'. Various RNA termini representing RNA processing sites at several parts of the 5S rRNA-tRNA(Arg) area were detected. This gene spacer is substantially conserved among various species compared here, and a secondary structure model for this chloroplast region in canola applies to other plant sequences. The conservation of this intergenic sequence suggests a functional role, possibly by providing recognition structures for endogenous RNases involved in its maturing process.

MeSH terms

  • Base Sequence
  • Brassica / genetics*
  • Chloroplasts / genetics*
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Nucleic Acid Conformation
  • RNA Processing, Post-Transcriptional*
  • RNA, Plant / genetics
  • RNA, Plant / physiology
  • RNA, Ribosomal, 5S / genetics*
  • RNA, Transfer, Arg / chemistry
  • RNA, Transfer, Arg / genetics*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • DNA, Ribosomal
  • RNA, Plant
  • RNA, Ribosomal, 5S
  • RNA, Transfer, Arg

Associated data

  • GENBANK/AF242851