Format

Send to

Choose Destination
Nat Genet. 2000 Aug;25(4):375-6.

Hot-stop PCR: a simple and general assay for linear quantitation of allele ratios.

Author information

1
Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Erratum in

  • Nat Genet 2001 May;28(1):97.

Abstract

We have developed a simple, quantitative assay for measurement of allele ratios that circumvents the problem of heteroduplex formation skewing the results of restriction endonuclease digestion of PCR products. This assay, 'hot-stop PCR', involves addition of a radiolabelled PCR primer at the final cycle. We applied the assay to analysis of loss of imprinting (LOI) of the insulin-like growth factor II gene (IGF2) in tumours.

PMID:
10932175
DOI:
10.1038/78040
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center