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Biosci Biotechnol Biochem. 2000 Jun;64(6):1203-9.

Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12.

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1
Department of Agricultural Chemistry, Kinki University, Nara, Japan.

Abstract

Spontaneous mutations have been isolated in Escherichia coli that result in the constitutive expression of an emrKY promoter. These mutations were found to be single-nucleotide substitutions within the linker region of the sensor protein EvgS, which is part of a two-component regulatory system along with EvgA. In the linker mutants (evgSI and evgS4), emrKY expression became constitutive and MIC against sodium deoxycholate was 20 mg/ml, eight-fold higher than in the wild type. Furthermore, the start site of transcription from the promoter of emrKY was identified; EvgA was shown to bind at the -52 to -84 region by the footprinting experiment.

PMID:
10923791
DOI:
10.1271/bbb.64.1203
[Indexed for MEDLINE]
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