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Biochim Biophys Acta. 1975 Apr 2;383(4):457-63.

A simple method for the preparation of large quantities of pure plasmid DNA.

Abstract

Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6, from cleared lysates of plasmid-carrying bacterial strains, After resuspension and density-gradient centrifugation of the precipitated DNA, it is unchanged in length and in transformation efficiency for Escherichia coli K12. Plasmid DNA can be easily prepared in large quantities by including a polyethylene glycol precipitation step in standard plasmid isolation procedures.

PMID:
1092355
DOI:
10.1016/0005-2787(75)90318-4
[Indexed for MEDLINE]

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