Enhanced binding of aminoglycoside dimers to a "dimerized" A-site 16S rRNA construct

Bioorg Med Chem Lett. 2000 Jul 17;10(14):1593-5. doi: 10.1016/s0960-894x(00)00299-7.

Abstract

In this work, we investigated the binding of a series of dimeric aminoglycoside molecules to (i) a 27 nt A-site 16S rRNA construct, and (ii) an artificially grafted 46 nt 'dimerized' A-site 16S rRNA construct. It was observed that the dissociation constants of dimeric aminoglycosides to the dimerized A-site 16S rRNA construct can achieve up to approximately 19-fold enhancement compared to the monomeric aminoglycoside molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides / chemical synthesis
  • Aminoglycosides / chemistry*
  • Anti-Bacterial Agents / chemical synthesis
  • Anti-Bacterial Agents / chemistry*
  • Binding Sites
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Dimerization
  • Molecular Conformation
  • Molecular Sequence Data
  • Molecular Structure
  • RNA, Ribosomal, 16S / chemistry*

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • RNA, Ribosomal, 16S