Abstract
In this work, we investigated the binding of a series of dimeric aminoglycoside molecules to (i) a 27 nt A-site 16S rRNA construct, and (ii) an artificially grafted 46 nt 'dimerized' A-site 16S rRNA construct. It was observed that the dissociation constants of dimeric aminoglycosides to the dimerized A-site 16S rRNA construct can achieve up to approximately 19-fold enhancement compared to the monomeric aminoglycoside molecules.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoglycosides / chemical synthesis
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Aminoglycosides / chemistry*
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Anti-Bacterial Agents / chemical synthesis
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Anti-Bacterial Agents / chemistry*
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Binding Sites
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Carbohydrate Conformation
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Carbohydrate Sequence
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Dimerization
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Molecular Conformation
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Molecular Sequence Data
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Molecular Structure
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RNA, Ribosomal, 16S / chemistry*
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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RNA, Ribosomal, 16S