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FEMS Microbiol Lett. 2000 Aug 1;189(1):15-8.

Identification of type 4 fimbriae in Actinobacillus pleuropneumoniae.

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CSIRO Animal Health, Australian Animal Health Laboratory, Private Bag 24, 3220, Geelong, Vic., Australia.


Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N-terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins.

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