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Med Wieku Rozwoj. 1999 Jan-Mar;3(1):171-9.

[Use of polymerase chain reaction for pediatric diagnosis].

[Article in Polish]

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1
Zakład Genetyki Medycznej AM w Lublinie.

Abstract

Basic principles of main molecular biology techniques: polymerase chain reaction (PCR) and its variants; asymmetric PCR, ARMS, nested PCR, multiplex PCR and competitive PCR are discussed. Nucleic acids techniques are making increasing progress in molecular diagnostics. Sequence analysis of amplified DNA allows better identification of the pathogen, detection of mutant genes and more accurate prognosis and therapy of certain diseases.

PMID:
10910648
[Indexed for MEDLINE]
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