Abstract
Excitatory amino acids which promote the survival of cerebellar granule cells in culture, also promote the expression of the survival of motor neuron (SMN) protein. Immunolocalization studies using SMN monoclonal antibody showed that SMN is decreased in cultures grown in low K+ or chemically defined medium with respect to cultures grown in high K+ medium and that an increase of SMN can be induced by treatment of low K+ cultures with glutamate or N-methyl-D-aspartate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Survival
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Cells, Cultured
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Cerebellum / drug effects
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Cerebellum / metabolism*
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Culture Media
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Cyclic AMP Response Element-Binding Protein
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Excitatory Amino Acids / metabolism*
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Fluorescent Antibody Technique
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Glutamic Acid / pharmacology
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Humans
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Microscopy, Fluorescence
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N-Methylaspartate / pharmacology
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Nerve Tissue Proteins / biosynthesis*
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Neurons / metabolism
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Potassium / pharmacology
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RNA-Binding Proteins
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Rats
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SMN Complex Proteins
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Survival of Motor Neuron 1 Protein
Substances
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Culture Media
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Cyclic AMP Response Element-Binding Protein
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Excitatory Amino Acids
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Nerve Tissue Proteins
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RNA-Binding Proteins
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SMN Complex Proteins
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Smn1 protein, rat
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Survival of Motor Neuron 1 Protein
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Glutamic Acid
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N-Methylaspartate
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Potassium