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Immunity. 2000 Jun;12(6):643-52.

Cell-type-restricted binding of the transcription factor NFAT to a distal IL-4 enhancer in vivo.

Author information

1
Department of Pathology, Harvard Medical School and the Center for Blood Research, Boston, Massachusetts 02115, USA.

Abstract

By DNase I hypersensitivity analysis, we have identified an inducible, cyclosporin A-sensitive enhancer located 3' of the interleukin-4 (IL-4) gene. The enhancer binds the Th2-specific transcription factor GATA3 in vivo but is not perceptibly influenced by the absence of a second Th2-specific factor, cMaf. The antigen-inducible transcription factor NFAT1 binds the IL-4 enhancer and the IL-4 promoter only in stimulated Th2 cells; conversely, NFAT1 binds to the interferon (IFN)-gamma promoter only in stimulated Th1 cells. Our results support a model whereby transcription factors such as NFAT1, which are nonselectively induced in antigen-stimulated T cells, gain access to cytokine regulatory regions only in the appropriate subset of differentiated T cells in vivo. This restricted access enables antigen-dependent and subset-specific transcription of cytokine genes.

PMID:
10894164
DOI:
10.1016/s1074-7613(00)80215-0
[Indexed for MEDLINE]
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