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Electrophoresis. 2000 Jun;21(11):2105-14.

Mass spectrometry: a tool for the identification of proteins separated by gels.

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F. Hoffmann-LaRoche Ltd., Pharmaceutical Research, Roche Genetics, Basel, Switzerland.


Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high-resolution two-dimensional (2-D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments during the last five years in MS associated with protein gel separation are reviewed.

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