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Cell. 2000 Jun 9;101(6):601-11.

Structural organization of the RNA polymerase-promoter open complex.

Author information

1
Howard Hughes Medical Institute, Department of Chemistry, Rutgers University, Piscataway, New Jersey 08854, USA.

Abstract

We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RPo). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of RPo, and permit analysis of effects of a transcriptional activator on the structure of RPo.

PMID:
10892647
DOI:
10.1016/s0092-8674(00)80872-7
[Indexed for MEDLINE]
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