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Nat Biotechnol. 2000 Jul;18(7):764-7.

Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell.

Author information

1
Advanced Monitoring Development Group, Life Sciences Division, P.O. Box 2008, Oak Ridge National Laboratory, Oak Ridge TN 37831-6101, USA.

Abstract

We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and of the BaP-DNA adduct. Detection of BPT is of great biomedical interest, since this species can serve as a biomarker for monitoring DNA damage due to BaP exposure and for possible precancer diagnosis. The measurements were performed on the rat liver epithelial clone 9 cell line, which was used as the model cell system. Before making measurements, the cells were treated with BPT. Nanoprobes were inserted into individual cells, incubated 5 min to allow antigen-antibody binding, and then removed for fluorescence detection. We determined a concentration of 9.6+/-0.2x10(-11) M for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe.

PMID:
10888846
DOI:
10.1038/77337
[Indexed for MEDLINE]

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