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Mamm Genome. 2000 Jul;11(7):572-6.

Cryopreservation of mouse spermatozoa.

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Division of Reproductive Engineering, Center for Animal Resources & Development, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.


Recently, it has become possible to freeze a large number of mouse spermatozoa immediately after collection from the epididymides of a small number of males. The cryopreservation of spermatozoa is simpler, less time-consuming, and less costly than that of embryos for maintaining various strains of mice with induced mutations. This chapter attempts to provide a realistic overview of the cryopreservation of mouse spermatozoa and to describe a detailed procedure for mouse sperm freezing.

[Indexed for MEDLINE]

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