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Dev Biol. 2000 Jun 1;222(1):124-34.

Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.

Author information

1
Department of Genetics, Cell and Developmental Biology, University of Minnesota, Minneapolis 55455, USA. heasman@lenti.med.umn.edu

Abstract

Xenopus embryos develop dorsal/ventral and anterior/posterior axes as a result of the activity of a maternal Xwnt pathway, in which beta-catenin is an essential component, acting as a transactivator of transcription of zygotic genes. However, the questions of where and when beta-catenin is required in early embryogenesis have not been addressed directly, because no loss-of-function method has been available. Here we report the use of a novel antisense approach that allows us to target depletion of protein to individual blastomeres. When a "morpholino" oligo complementary to beta-catenin mRNA is injected into early embryos, it depletes beta-catenin protein effectively through the neurula stage. By targeting the oligo to different cleavage blastomeres, we block beta-catenin activity in different areas and at different times. Dorsal vegetal injection at the 2- and 4-cell stages blocks dorsal axis formation and at the 8-cell stage blocks head formation, while A-tier injection at the 32-cell stage causes abnormal cement gland formation. This approach shows the complex involvement of Xwnt pathways in embryonic patterning and offers a rapid method for the functional analysis of both maternal and early zygotic gene products in Xenopus.

PMID:
10885751
DOI:
10.1006/dbio.2000.9720
[Indexed for MEDLINE]
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