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Clin Diagn Lab Immunol. 2000 Jul;7(4):714-6.

Viability and functional activity of cryopreserved mononuclear cells.

Author information

1
University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. adriana.weinberg@uchsc.edu

Abstract

Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte proliferation, was associated with viability. Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses.

PMID:
10882680
PMCID:
PMC95942
DOI:
10.1128/cdli.7.4.714-716.2000
[Indexed for MEDLINE]
Free PMC Article

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