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Methods. 2000 Jul;21(3):249-57.

Analysis of virus-infected cells by flow cytometry.

Author information

1
Department of Microbiology, Immunology and Molecular Genetics, Mail Code 68, 47 New Scotland Avenue, Albany, New York, 12208, USA.

Abstract

Flow cytometry has been used to study virus-cell interactions for many years. This article critically reviews a number of reports on the use of flow cytometry for the detection of virus-infected cells directly in clinical samples and in virus-infected cultured cells. Examples are presented of the use of flow cytometry to screen antiviral drugs against human immunodeficiency virus (HIV), human cytomegalovirus, and herpes simplex viruses (HSV) and to perform drug susceptibility testing for these viruses. The use of reporter genes such as green fluorescent protein incorporated into HIV or HSV or into cells for the detection of the presence of virus, for drug susceptibility assay, and for viral pathogenesis is also covered. Finally, studies on the use of flow cytometry for studying the effect of virus infection on apoptosis and the cell cycle are summarized. It is hoped that this article will give the reader some understanding of the great potential of this technology for studying virus cell interactions.

PMID:
10873479
DOI:
10.1006/meth.2000.1005
[Indexed for MEDLINE]

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