Abstract
Mammalian kinetochores contain the centromere-specific histone H3 variant CENP-A, whose incorporation into limited chromosomal regions may be important for centromere function and chromosome segregation during mitosis. However, regulation of CENP-A localization and its role have not been clear. Here we report that the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation. SpCENP-A binding to the nonrepetitious inner centromeres depended on Mis6, an essential centromere connector protein acting during G1-S phase of the cell cycle. Mis6 is likely required for recruiting SpCENP-A to form proper connection of sister centromeres.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cell Cycle Proteins / metabolism
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Cell Cycle*
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Centromere / metabolism*
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Chromatin / metabolism
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Chromosomal Proteins, Non-Histone / chemistry
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism*
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Chromosome Segregation*
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DNA Replication
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DNA, Fungal / metabolism
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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G1 Phase
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Histones / chemistry
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Histones / genetics
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Histones / metabolism
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Mitosis
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Molecular Sequence Data
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Mutation
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Recombinant Fusion Proteins / metabolism
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S Phase
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Schizosaccharomyces / cytology
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism*
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Schizosaccharomyces pombe Proteins*
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Temperature
Substances
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Cell Cycle Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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Cnp1 protein, S pombe
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DNA, Fungal
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Fungal Proteins
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Histones
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Mis6 protein, S pombe
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Recombinant Fusion Proteins
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Schizosaccharomyces pombe Proteins
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mis12 protein, S pombe