Matrix-assisted laser desorption/ionization mass spectrometric quantification of the mu opioid receptor agonist DAMGO in ovine plasma

J Mass Spectrom. 2000 Jun;35(6):725-33. doi: 10.1002/1096-9888(200006)35:6<725::AID-JMS1>3.0.CO;2-I.

Abstract

The synthetic opioid peptide analog Tyr-D-Ala-Gly-N-methyl-Phe-Gly-ol (DAMGO), which is a mu opioid receptor-selective agonist, was quantified in ovine plasma samples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), using delayed extraction and a reflectron. The internal standard was pentadeuterated DAMGO. Timed-ion selection was used to select the precursor ion. The analysis of the post-source decay fragments improved the detection sensitivity, and the use of the precursor-product ion relationship optimized the specificity. For plasma samples, the inter-assay variability of this method was 6.4% (n = 79) and the intra-assay variability was 6.0% (n = 10). The variability for controls was 3.4% (n = 43). The profile of DAMGO amount versus time was determined in sheep plasma, and the corresponding pharmacokinetic data were calculated.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Analysis of Variance
  • Animals
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / administration & dosage
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / blood*
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / pharmacokinetics
  • Female
  • Infusions, Intravenous
  • Receptors, Opioid, mu / agonists*
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sheep
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / standards
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / statistics & numerical data

Substances

  • Receptors, Opioid, mu
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)-