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Yeast. 2000 Jun 30;16(9):857-60.

Rapid and reliable protein extraction from yeast.

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1
Institute of Experimental Cardiology, Cardiology Research Centre, Moscow, Russia. vita@cardio.ru

Abstract

The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates.

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