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Inflamm Res. 2000 Apr;49(4):170-6.

Participation of endogenous endothelins in delayed eosinophil and neutrophil recruitment in mouse pleurisy.

Author information

1
Laboratório de Farmacologia Aplicada, Instituto de Tecnologia em Fármacos - FARMANGUINHOS - Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.

Abstract

OBJECTIVE:

Investigate the role of endothelins in leukocyte recruitment in allergic and non allergic inflammation.

METHODS:

Pleurisy was induced in mice by intrathoracic injection of ovalbumin (OVA; in sensitized animals), E. coli LPS, carrageenan, Mycobacterium bovis (BCG) or zymosan. Animals were treated with BQ-123 or BQ-788 (1.5-150 pmol/cavity), or intravenously with bosentan (30 mg/kg).

RESULTS:

None of the ET receptor antagonists modified early neutrophil recruitment (at 4 h) induced by OVA, LPS, carrageenan, BCG or zymosan or plasma leakage caused by carrageenan or zymosan. Mononuclear and eosinophil accumulation triggered by OVA were reduced by BQ-123 (150 pmol/cavity) or bosentan (68 and 43% inhibition of eosinophilia), but unaffected by BQ-788. BQ-123 and bosentan also inhibited LPS increases in neutrophil (by 67 and 40%) and eosinophil (by 63 and 74%) at 24 h.

CONCLUSIONS:

Endothelins, acting via ETA receptors, play a role in late eosinophil and neutrophil accumulation (24 h), but not in the acute (4 h) neutrophilic response.

PMID:
10858017
DOI:
10.1007/s000110050577
[Indexed for MEDLINE]

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