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Trends Cell Biol. 2000 Jul;10(7):290-5.

Protein transduction: unrestricted delivery into all cells?

Author information

1
Howard Hughes Medical Institute, Depts of Pathology and Medicine, and Barnes-Jewish Hospital, Washington University School of Medicine, 4940 Parkview Place, Campus Box 8022, St Louis, MO 63110, USA.

Abstract

Several proteins can traverse biological membranes through protein transduction. Small sections of these proteins (10-16 residues long) are responsible for this. Linking these domains covalently to compounds, peptides, antisense peptide nucleic acids or 40-nm iron beads, or as in-frame fusions with full-length proteins, lets them enter any cell type in a receptor- and transporter-independent fashion. Moreover, several of these fusions, introduced into mice, were delivered to all tissues, even crossing the blood-brain barrier. These domains thus might let us address new questions and even help in the treatment of human disease.

PMID:
10856932
DOI:
10.1016/s0962-8924(00)01771-2
[Indexed for MEDLINE]

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