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Fertil Steril. 2000 Jun;73(6):1226-31.

Seminal plasma characteristics as indicators of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in men with obstructive azoospermia.

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1
Institutes of Reproductive Medicine and Human Genetics of the University, M√ľnster, Germany.

Abstract

OBJECTIVE:

To determine the prognostic value of seminal plasma volume, pH, fructose, and alpha-glucosidase for the detection of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations.

DESIGN:

Retrospective data analysis.

SETTING:

University infertility clinic (referral center).

PATIENT(S):

Fifty-nine men with obstructive azoospermia.

INTERVENTION(S):

Semen analysis including seminal plasma volume, pH, fructose, alpha-glucosidase, molecular genetic diagnosis of CFTR mutations and FSH measurement.

MAIN OUTCOME MEASURE(S):

Sensitivity and specificity of seminal plasma markers for the detection of CFTR mutations.

RESULT(S):

A CFTR mutation was detected in 26 of 59 patients with obstructive azoospermia. Patients carrying a mutation had significantly lower seminal plasma volume (mean +/- SEM: 1.5 +/- 1.4 mL vs. 2.8 +/- 2.2 mL), lower pH levels (25th percentile, median, 75th percentile: 6.5, 6.8, 7.5 vs. 7.7, 7. 9, 7.9) and lower fructose content (1.0, 1.1, 3.7 vs. 5.8, 20.0, 83. 0 micromol/ejaculate) than those without mutations. Diagnostic efficacy for detection of mutations was best (pH 81.4%, fructose 81. 8%) at a cutoff level for pH of 7.4 and fructose of 2 micromol/ejaculate.

CONCLUSION(S):

Seminal plasma markers provide an effective, noninvasive method to predict CFTR mutations in men with obstructive azoospermia.

PMID:
10856487
[Indexed for MEDLINE]
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