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Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7382-7.

A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions.

Author information

1
Howard Hughes Medical Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

We have developed a bacterial "two-hybrid" system that readily allows selection from libraries larger than 10(8) in size. Our bacterial system may be used to study either protein-DNA or protein-protein interactions, and it offers a number of potentially significant advantages over existing yeast-based one-hybrid and two-hybrid methods. We tested our system by selecting zinc finger variants (from a large randomized library) that bind tightly and specifically to desired DNA target sites. Our method allows sequence-specific zinc fingers to be isolated in a single selection step, and thus it should be more rapid than phage display strategies that typically require multiple enrichment/amplification cycles. Given the large library sizes our bacterial-based selection system can handle, this method should provide a powerful tool for identifying and optimizing protein-DNA and protein-protein interactions.

PMID:
10852947
PMCID:
PMC16554
DOI:
10.1073/pnas.110149297
[Indexed for MEDLINE]
Free PMC Article

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