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FEBS Lett. 2000 Jun 2;474(2-3):159-64.

Inactivation of p53 and life span extension of human diploid fibroblasts by mot-2.

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National Institute of Bioscience and Human Technology, AIST, Tsukuba, Ibaraki, Japan.


Normal human lung fibroblasts were transfected with expression plasmids encoding mot-2, an hsp70 family member that is associated with the immortal phenotype. After the empty vector-transfected controls had become senescent and positive for senescence-associated beta-galactosidase (SA-beta-gal), the mot-2-expressing cells continued to proliferate for an additional 12-18 population doublings and showed a young cell morphology and much lower SA-beta-gal activity. The tumor suppressor p53 was found to be transcriptionally inactivated in life span-extended cells. We have thus shown for the first time that overexpression of mot-2 in normal human cells is able to permit their temporary escape from senescence.

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