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Curr Opin Plant Biol. 2000 Jun;3(3):224-8.

Unraveling plant metabolism by EST analysis.

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Department of Botany and Plant Pathology, Michigan State University, East Lansing 48823, USA.


Large-scale single-pass sequencing of cDNAs prepared from specific plant species or tissues has evolved as an inexpensive and efficient gene-discovery tool that can be used to identify novel cDNAs encoding enzymes of specific plant metabolic pathways. Collections of expressed sequence tags from metabolically active tissues can provide quantitative estimates of gene expression levels and thus are being exploited to unravel plant metabolic and regulatory networks.

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