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J Biol Chem. 1979 May 25;254(10):4063-71.

Structure of the oligosaccharide of human J chain.

Abstract

The complete structure of the oligosaccharide moiety of J chain isolated from a Waldenströms macroglobulin Wa has been established. The oligosaccharide is present in three forms differing in the amount of sialic acid and designated J-A, J-B, and J-C. The structure and proportion of each of these is: formula : (see text) : Removal of the oligosaccharide moiety with glycosidases results in an increased mobility of J chain in sodium dodecyl sulfate polyacrylamide gel electrophoresis corresponding to a shift in apparent molecular weight from 23,500 to 19,500. The preparation and utilization of iodinated glycopeptides for sequence analysis is presented.

PMID:
108268
[Indexed for MEDLINE]
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