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Biochem Biophys Res Commun. 2000 May 19;271(3):615-9.

Putative P-glycoprotein expression in arsenite-resistant Leishmania donovani down-regulated by verapamil.

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Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Punjab, 160 062, India.


Western immunoblots of whole cell lysate and crude membrane extract of an in vitro selected sodium m-arsenite-resistant L. donovani strain revealed a 230-kDa protein identified by an anti-P-glycoprotein (Pgp) antibody. Immunofluorescence microscopy, using the same antibody, detected putative Pgp on resistant parasites. Overexpression of the putative Pgp was down-regulated by verapamil. These results provided, possibly, the first evidence that (i) overexpression of Pgp-like protein occurs in arsenite-resistant Leishmania that are cross-resistant to structurally and functionally unrelated drugs and (ii) verapamil regulates drug sensitivity possibly by down-regulating Pgp expression in the arsenite-resistant Leishmania.

[Indexed for MEDLINE]

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