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Biotech Histochem. 2000 Jan;75(1):23-6.

Embedding thin plant specimens for oriented sectioning.

Author information

1
Department of Genetics, Flanders Interuniversitary Institute for Biotechnology, University of Gent, Belgium. tobee@gengenp.rug.ac.be

Abstract

Small plant structures such as small primary roots, filamentous mosses and algae are difficult to orient for sectioning since they become wavy and curl during embedding. A method is described for embedding and orienting tiny plant specimens in a glycol methacrylate resin using self-constructed flat molds. Prior to sectioning, small samples can be oriented in both the longitudinal and the transverse plane. As several samples can be sectioned simultaneously, time-consuming trimming of the blocks is reduced substantially. The efficiency of this technique has been demonstrated using the tiny roots of the model plant Arabidopsis thaliana (L.) Heynh.

PMID:
10810979
[Indexed for MEDLINE]

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