Format

Send to

Choose Destination
Nat Struct Biol. 2000 May;7(5):394-7.

Two energetically disparate folding pathways of alpha-lytic protease share a single transition state.

Author information

1
The Howard Hughes Medical Institute and the Department of Biochemistry and Biophysics, University of California, San Francisco, 513 Parnassus Avenue, Box 0448, San Francisco, California 94143-0448, USA.

Abstract

The Lysobacter enzymogenes alpha-lytic protease (alphaLP) is synthesized with a 166 amino acid pro region (Pro) that catalyzes the folding of the 198 amino acid protease into its native conformation. An extraordinary feature of this system is the very high energy barrier (DeltaG = 30 kcal mol-1) that effectively prevents alphaLP from folding in the absence of Pro (t1/2 = 1800 years). A pair of mutations has been isolated in the protease that completely suppresses the catalytic defect incurred in Pro by truncation of its last three amino acids. These mutations also accelerate the folding of alphaLP in the absence of Pro by 400-fold. An energetic analysis of the two folding reactions indicates that the mutations stabilize the transition states of both the catalyzed and uncatalyzed folding reactions by 3 kcal mol-1. This finding points to a single transition state for these two distinct and energetically disparate folding pathways, and raises the possibility that all alphaLP folding pathways share the same transition state.

PMID:
10802737
DOI:
10.1038/75172
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center