Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2000 May 10;271(2):418-21.

Neuronal differentiation of cryopreserved neural progenitor cells derived from mouse embryonic stem cells.

Author information

1
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, 30912, USA.

Abstract

Embryonic stem cells (ES cells) are developmentally pluripotent cells isolated from pre-implantation mammalian embryos. In cell culture ES cells can be easily differentiated to generate cultures of neural progenitors. We present a simple method for the cryopreservation of these ES-derived neural progenitors. Cryopreserved neural progenitor stocks can be thawed, expanded with FGF2, and differentiated into functional neurons. This method will facilitate studies using ES-derived neural progenitor cells as a cell culture model system for neural development and differentiation. It will also aid studies designed to test the ability of these progenitor cells to functionally engraft and repair damaged neural tissue.

PMID:
10799312
DOI:
10.1006/bbrc.2000.2631
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center