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Planta. 2000 Mar;210(4):599-606.

Salicylic acid-independent induction of pathogenesis-related gene expression by fusicoccin.

Author information

1
Institute of Plant Sciences, ETH-Z├╝rich, Switzerland. andreas.schaller@ipw.biol.ethz.ch

Abstract

Treatment of tomato plants (Lycopersicon esculentum Mill.) with fusicoccin (FC), an activator of the plasma-membrane H+-ATPase which maintains an electrochemical gradient across the plasma membrane, resulted in a dose-dependent accumulation of transcripts for intra- and extracellular pathogenesis-related (PR) proteins. The accumulation of PR protein transcripts was paralleled by an increase in leaf salicylic acid (SA) content. Transcripts of PR proteins and SA started to accumulate 3 h after FC treatment. 2-Aminoindan-2-phosphonic acid, an inhibitor of SA synthesis, was used to assess the role of SA in FC-mediated induction of PR gene expression. 2-Aminoindan-2-phosphonic acid was found to suppress the accumulation of SA but not the induction of PR gene expression in response to FC treatment. Furthermore, in transgenic tobacco plants overexpressing a bacterial salicylate hydroxylase gene (nahG-tobacco), PR transcripts accumulated after FC treatment to levels similar to those observed in control tobacco plants. The data indicate a role for the proton gradient across the plasma membrane in the SA-independent induction of PR gene expression.

PMID:
10787053
DOI:
10.1007/s004250050049
[Indexed for MEDLINE]

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