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Electrophoresis. 2000 Apr;21(6):1054-70.

Membrane proteins and proteomics: un amour impossible?

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INRA, Laboratoire de biochemie et physiologie mol├ęculaire des plantes, Montpellier, France.


Proteome analysis implies the ability to separate proteins as a first step prior to characterization. Thus, the overall performance of the analysis strongly depends on the performance of the separation tool, usually two-dimensional electrophoresis. This review shows how two-dimensional electrophoresis performs with membrane proteins from bacteria or animal or vegetable cells and tissues, the recent progress in this field, and it examines future prospects in this area.

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