Abstract
SnR, twist and Fgf10 are expressed in presumptive limb territories of early chick embryos. When FGF-2/FGF-8 beads are implanted in chick flank, an ectopic limb develops and SnR is irreversibly activated as early as 1 h. Ectopic Fgf10 and twist expression are activated much later at 17 and 20 h, respectively. FGF-10 can also induce SnR, but much later, and in this case activation occurs simultaneously with that of twist and Fgf10 via the Fgf8- expressing ridge. Tbx-4 and Tbx-5 are expressed in leg and wing forming regions, respectively, in a similar pattern to SnR and twist. FGF-2 leads to ectopic expression of Tbx-4 and Tbx-5 as rapidly as ectopic expression of SnR, but the patterns of ectopic transcripts suggest that induction of SnR and Tbx gene expression occur via different pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Avian Proteins*
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Base Sequence
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Chick Embryo
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DNA, Complementary
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DNA-Binding Proteins / genetics*
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Extremities / embryology
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Fibroblast Growth Factor 10
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Fibroblast Growth Factor 2 / pharmacology
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Fibroblast Growth Factor 8
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Fibroblast Growth Factors / genetics*
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Fibroblast Growth Factors / pharmacology
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Gene Expression Regulation, Developmental* / drug effects
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Molecular Sequence Data
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Snail Family Transcription Factors
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T-Box Domain Proteins / genetics*
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Transcription Factors / genetics*
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Twist-Related Protein 1
Substances
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Avian Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Fibroblast Growth Factor 10
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Snail Family Transcription Factors
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T-Box Domain Proteins
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T-box transcription factor 5
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TBX4 protein, Gallus gallus
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Transcription Factors
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Twist protein, Gallus gallus
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Twist-Related Protein 1
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snail-related protein, Gallus gallus
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Fibroblast Growth Factor 2
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Fibroblast Growth Factor 8
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Fibroblast Growth Factors